Production of Purified MRSAcin from Local Clinical Strain of Methicillin-Resistant Staphylococcus aureus and its Applicability as Antimicrobial

Strains MRSA that acquired a specific gene makes for resistant to most (beta-lactam) antibiotics. Also common resistance to other antibiotics especially in hospital-associated, these organisms microbiology are nosocomial pathogens serious and finding an effective treatment can be challenging. Community associated MRSA strains are outside hospitals originated having moved into hospitals and become resistant to drugs increasing than beta-lactams [1]. (Methicillin-resistant Staphylococcus aureus) resistant usually to several type antibiotics and also resistance to ßlactam and a particular shows ability to spread in most hospitals and present now in most countries of Iraq [2]. Risk factors for the hospital acquisition of MRSA include prolonged hospitalization that stay in an intensive care unit, chronic diseases such as chronic renal failure and malignancy, prior exposure to antibiotics, surgery and contact with a patient known to be colonized or infected with MRSA [3]. Bacteriocins are antimicrobial peptides or proteins ribosomally safe substances that are non-toxic to eukaryotic cells. Bacteriocins bacterially produced natural peptides released by different varieties of bacteria and archea that are active against other bacteria and the producer has a specific immunity mechanism [4]. Bacteriocins which have inhibitory effects towards sensitive strains are produced by both gram-positive and gram-negative bacteria, Bacteriocins from lactic acid bacteria are used in food perecierpitive like in dairy products. It has a useful use in health care products and cosmetics for treatment of acne, also being used inhibition of dental caries in toothpaste and mouthwash for the periodontal diseases [5]. Staphylococcin Bac188 also showed very potent activity against many clinical isolates of Mycobacterium tuberculosis. Staphylococcin Bac188) Staphylococci are known to produce a wide variety of inhibitory substances [6]. Other antimicrobial substances produced by staphylococci have been categorized as true bacteriocins [7].